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PKM2 promotes stemness of breast cancer cell by through Wnt/β-catenin pathway.
PKM2 promotes stemness of breast cancer cell by through Wnt/β-catenin pathway.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2016 Mar; Vol. 37 (3), pp. 4223-34. Date of Electronic Publication: 2015 Oct 22. - Publication Year :
- 2016
-
Abstract
- Metastasis is one of the main causes of breast cancer (BCa)-related deaths in female. It has been reported that cancer stem cell played an important role in metastasis. Here we first revealed a specific role of pyruvate kinase isozymes M2 (PKM2) in the stemness of breast cancer cells. Breast cancer tissue analysis confirmed the upregulation of PKM2 in breast cancer, and high PKM2 levels were associated with poor prognosis of breast cancer patients. Holoclone assay and colony formation assay significantly elucidated the role of PKM2 in the self-renewal of breast cancer cells. Moreover, PKM2 elevated the proportion of stem cell and the ability of sphere formation in breast cancer cells. PKM2 played its functional role in stemness by regulating β-catenin. Collectively, we identified critical roles of PKM2 in the stemness of breast cancer cells which may elevate the therapeutic effect on breast cancer patients.
- Subjects :
- Breast Neoplasms mortality
Breast Neoplasms pathology
Cell Line, Tumor
Cell Self Renewal
Female
Gene Expression
Humans
Isoenzymes physiology
Neoplasm Metastasis
Prognosis
beta Catenin genetics
beta Catenin metabolism
Breast Neoplasms enzymology
Neoplastic Stem Cells enzymology
Pyruvate Kinase physiology
Wnt Signaling Pathway
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26493994
- Full Text :
- https://doi.org/10.1007/s13277-015-4121-8