Back to Search
Start Over
A Genetic Cascade of let-7-ncl-1-fib-1 Modulates Nucleolar Size and rRNA Pool in Caenorhabditis elegans.
- Source :
-
PLoS genetics [PLoS Genet] 2015 Oct 22; Vol. 11 (10), pp. e1005580. Date of Electronic Publication: 2015 Oct 22 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Ribosome biogenesis takes place in the nucleolus, the size of which is often coordinated with cell growth and development. However, how metazoans control nucleolar size remains largely unknown. Caenorhabditis elegans provides a good model to address this question owing to distinct tissue distribution of nucleolar sizes and a mutant, ncl-1, which exhibits larger nucleoli than wild-type worms. Here, through a series of loss-of-function analyses, we report that the nucleolar size is regulated by a circuitry composed of microRNA let-7, translation repressor NCL-1, and a major nucleolar pre-rRNA processing protein FIB-1/fibrillarin. In cooperation with RNA binding proteins PUF and NOS, NCL-1 suppressed the translation of FIB-1/fibrillarin, while let-7 targeted the 3'UTR of ncl-1 and inhibited its expression. Consequently, the abundance of FIB-1 is tightly controlled and correlated with the nucleolar size. Together, our findings highlight a novel genetic cascade by which post-transcriptional regulators interplay in developmental control of nucleolar size and function.
- Subjects :
- 3' Untranslated Regions
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins metabolism
Carrier Proteins metabolism
Cell Nucleolus genetics
Cell Size
Chromosomal Proteins, Non-Histone metabolism
Female
MicroRNAs metabolism
Optical Imaging
RNA-Binding Proteins genetics
RNA-Binding Proteins metabolism
Ribosomal Proteins metabolism
Ribosomes metabolism
Vulva growth & development
Vulva metabolism
Caenorhabditis elegans Proteins genetics
Carrier Proteins genetics
Chromosomal Proteins, Non-Histone genetics
MicroRNAs genetics
RNA, Ribosomal genetics
Ribosomal Proteins genetics
Ribosomes genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 11
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 26492166
- Full Text :
- https://doi.org/10.1371/journal.pgen.1005580