Back to Search
Start Over
Comparative Adjuvant Effects of Type II Heat-Labile Enterotoxins in Combination with Two Different Candidate Ricin Toxin Vaccine Antigens.
- Source :
-
Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2015 Dec; Vol. 22 (12), pp. 1285-93. Date of Electronic Publication: 2015 Oct 21. - Publication Year :
- 2015
-
Abstract
- Type II heat-labile enterotoxins (HLTs) constitute a promising set of adjuvants that have been shown to enhance humoral and cellular immune responses when coadministered with an array of different proteins, including several pathogen-associated antigens. However, the adjuvant activities of the four best-studied HLTs, LT-IIa, LT-IIb, LT-IIb(T13I), and LT-IIc, have never been compared side by side. We therefore conducted immunization studies in which LT-IIa, LT-IIb, LT-IIb(T13I), and LT-IIc were coadministered by the intradermal route to mice with two clinically relevant protein subunit vaccine antigens derived from the enzymatic A subunit (RTA) of ricin toxin, RiVax and RVEc. The HLTs were tested with low and high doses of antigen and were assessed for their abilities to stimulate antigen-specific serum IgG titers, ricin toxin-neutralizing activity (TNA), and protective immunity. We found that all four HLTs tested were effective adjuvants when coadministered with RiVax or RVEc. LT-IIa was of particular interest because as little as 0.03 μg when coadministered with RiVax or RVEc proved effective at augmenting ricin toxin-specific serum antibody titers with nominal evidence of local inflammation. Collectively, these results justify the need for further studies into the mechanism(s) underlying LT-IIa adjuvant activity, with the long-term goal of evaluating LT-IIa's activity in humans.<br /> (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Administration, Intranasal
Animals
Antibodies, Neutralizing blood
Antigens, Bacterial immunology
Bacterial Toxins administration & dosage
Enterotoxigenic Escherichia coli chemistry
Enterotoxins administration & dosage
Escherichia coli Proteins administration & dosage
Injections, Intradermal
Mice
Ricin chemistry
Vaccines administration & dosage
Adjuvants, Immunologic chemistry
Antigens, Bacterial administration & dosage
Bacterial Toxins immunology
Enterotoxins immunology
Escherichia coli Proteins immunology
Immunoglobulin G blood
Ricin immunology
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1556-679X
- Volume :
- 22
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Clinical and vaccine immunology : CVI
- Publication Type :
- Academic Journal
- Accession number :
- 26491037
- Full Text :
- https://doi.org/10.1128/CVI.00402-15