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Paper-Based Analytical Devices Relying on Visible-Light-Enhanced Glucose/Air Biofuel Cells.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2015 Nov 04; Vol. 7 (43), pp. 24330-7. Date of Electronic Publication: 2015 Oct 27. - Publication Year :
- 2015
-
Abstract
- A strategy that combines visible-light-enhanced biofuel cells (BFCs) and electrochemical immunosensor into paper-based analytical devices was proposed for sensitive detection of the carbohydrate antigen 15-3 (CA15-3). The gold nanoparticle modified paper electrode with large surface area and good conductibility was applied as an effective matrix for primary antibodies. The glucose dehydrogenase (GDH) modified gold-silver bimetallic nanoparticles were used as bioanodic biocatalyst and signal magnification label. Poly(terthiophene) (pTTh), a photoresponsive conducting polymer, served as catalyst in cathode for the reduction of oxygen upon illumination by visible light. In the bioanode, electrons were generated through the oxidation of glucose catalyzed by GDH. The amount of electrons is determined by the amount of GDH, which finally depended on the amount of CA15-3. In the cathode, electrons from the bioanode could combine with the generated holes in the HOMO energy level of cathode catalysts pTTh. Meanwhile, the high energy level photoexcited electrons were generated in the LUMO energy level and involved in the oxygen reduction reaction, finally resulting in an increasing current and a decreasing overpotential. According to the current signal, simple and efficient detection of CA15-3 was achieved.
- Subjects :
- Air
Antibodies chemistry
Biosensing Techniques methods
Catalysis
Electrochemistry methods
Electrodes
Electrons
Enzymes, Immobilized chemistry
Equipment Design
Gluconates chemistry
Gold
Immunoassay
Lactones chemistry
Light
Metal Nanoparticles chemistry
Mucin-1 chemistry
NAD chemistry
Oxygen chemistry
Photochemistry methods
Polymers chemistry
Thermoplasma enzymology
Thiophenes chemistry
Bioelectric Energy Sources
Biofuels
Glucose chemistry
Paper
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 7
- Issue :
- 43
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 26479328
- Full Text :
- https://doi.org/10.1021/acsami.5b07698