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Degradation of Benzene by Pseudomonas veronii 1YdBTEX2 and 1YB2 Is Catalyzed by Enzymes Encoded in Distinct Catabolism Gene Clusters.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2015 Oct 16; Vol. 82 (1), pp. 167-73. Date of Electronic Publication: 2015 Oct 16 (Print Publication: 2016). - Publication Year :
- 2015
-
Abstract
- Pseudomonas veronii 1YdBTEX2, a benzene and toluene degrader, and Pseudomonas veronii 1YB2, a benzene degrader, have previously been shown to be key players in a benzene-contaminated site. These strains harbor unique catabolic pathways for the degradation of benzene comprising a gene cluster encoding an isopropylbenzene dioxygenase where genes encoding downstream enzymes were interrupted by stop codons. Extradiol dioxygenases were recruited from gene clusters comprising genes encoding a 2-hydroxymuconic semialdehyde dehydrogenase necessary for benzene degradation but typically absent from isopropylbenzene dioxygenase-encoding gene clusters. The benzene dihydrodiol dehydrogenase-encoding gene was not clustered with any other aromatic degradation genes, and the encoded protein was only distantly related to dehydrogenases of aromatic degradation pathways. The involvement of the different gene clusters in the degradation pathways was suggested by real-time quantitative reverse transcription PCR.<br /> (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Bacterial Proteins genetics
Biocatalysis
Biodegradation, Environmental
Dioxygenases genetics
Dioxygenases metabolism
Molecular Sequence Data
Oxygenases genetics
Oxygenases metabolism
Phylogeny
Pseudomonas metabolism
Bacterial Proteins metabolism
Benzene metabolism
Multigene Family
Pseudomonas enzymology
Pseudomonas genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5336
- Volume :
- 82
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26475106
- Full Text :
- https://doi.org/10.1128/AEM.03026-15