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Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.

Authors :
Iino R
Sakakihara S
Matsumoto Y
Nishino K
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1333, pp. 101-9.
Publication Year :
2016

Abstract

A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.

Details

Language :
English
ISSN :
1940-6029
Volume :
1333
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
26468103
Full Text :
https://doi.org/10.1007/978-1-4939-2854-5_9