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Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2015 Oct 27; Vol. 112 (43), pp. 13378-83. Date of Electronic Publication: 2015 Oct 14. - Publication Year :
- 2015
-
Abstract
- An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.
- Subjects :
- Arabidopsis cytology
Cloning, Molecular
DNA Primers genetics
Germ Cells, Plant metabolism
Plasmids genetics
Protein Transport physiology
Real-Time Polymerase Chain Reaction
Arabidopsis physiology
Arabidopsis Proteins metabolism
Cell Communication physiology
Phosphoprotein Phosphatases metabolism
Pollen physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 112
- Issue :
- 43
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 26466609
- Full Text :
- https://doi.org/10.1073/pnas.1510854112