Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum.

Authors :: Collot M
Kreder R
Tatarets AL
Patsenker LD
Mely Y
Klymchenko AS
Source :: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2015 Dec 14; Vol. 51 (96), pp. 17136-9.
Publication Year :: 2015
Abstract: A rational design of squaraine dyes with lipophilic and zwitterionic groups tunes cell entry, allowing for selective far-red/near-infrared imaging of plasma membrane vs. endoplasmic reticulum. They exhibit up to 110-fold fluorescence enhancement in biomembranes and enable cellular imaging at 1 nM concentration, which make them the brightest membrane probes to date.

Subjects :: Cyclobutanes chemical synthesis
Fluorescence
Fluorescent Dyes chemical synthesis
HeLa Cells
Humans
Molecular Structure
Phenols chemical synthesis
Cell Membrane chemistry
Cyclobutanes chemistry
Endoplasmic Reticulum chemistry
Fluorescent Dyes chemistry
Phenols chemistry

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