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Expression of heat shock protein 27 and troponin T and troponin I after naloxone-precipitated morphine withdrawal.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2015 Nov 05; Vol. 766, pp. 142-50. Date of Electronic Publication: 2015 Oct 09. - Publication Year :
- 2015
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Abstract
- Heat shock protein (Hsp27) renders cardioprotection from stress situations but little is known about its role in myofilaments. In this study we have evaluated the relationship between Hsp27 and troponin response after naloxone-induced morphine withdrawal. Rats were treated with two morphine (75 mg) pellets during six days. Precipitated withdrawal was induced by naloxone on day seven. Hsp27 expression, Hsp27 phosphorylated at serine 82 (Ser82), cardiac troponin T (cTnT), cardiac troponin I (cTnI) and µ-calpain were evaluated by immunoblotting in left ventricle. Hsp, cTnT and cTnI was also evaluated by immunofluorescence procedure. Our results show that enhancement in Hsp27 expression and phosphorylation induced by naloxone-precipitated morphine withdrawal occurs with concomitant increases of cTnT and µ-calpain expression, whereas cTnI was decreased. We also observed co-localization of Hsp27 with cTnT in cardiac tissues. These findings provide new information into the possible role of Hsp27 in the protection of cTnT degradation by µ-calpain (a protease mediating proteolysis of cTnT and cTnI) after morphine withdrawal.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Analgesics, Opioid pharmacology
Animals
Arterial Pressure drug effects
Calpain metabolism
Heart Rate drug effects
Heart Ventricles metabolism
Male
Morphine pharmacology
Morphine Dependence physiopathology
Naloxone pharmacology
Narcotic Antagonists pharmacology
Phosphorylation drug effects
Rats, Sprague-Dawley
Substance Withdrawal Syndrome physiopathology
HSP27 Heat-Shock Proteins metabolism
Morphine Dependence metabolism
Substance Withdrawal Syndrome metabolism
Troponin I metabolism
Troponin T metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 766
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26452515
- Full Text :
- https://doi.org/10.1016/j.ejphar.2015.10.006