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Synthetically modified mRNA for efficient and fast human iPS cell generation and direct transdifferentiation to myoblasts.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2016 May 06; Vol. 473 (3), pp. 743-51. Date of Electronic Publication: 2015 Oct 09. - Publication Year :
- 2016
-
Abstract
- Synthetic mRNA transfection enables efficient and controlled gene expression in human cells, without genome integrations. Further, modifications to the mRNA and transfection protocol now allow for repeated transfection and long-term gene expression of an otherwise short-lived mRNA expression. This is mainly achieved through introducing modified nucleosides and interferon suppression. In this study we provide an overview and details of the advanced synthesis and modifications of mRNA originally developed for reprogramming. This mRNA allows for very efficient transfection of fibroblasts enabling the generation of high quality human iPS cells with a six-factor mRNA cocktail in 9 days. Furthermore, we synthesised and transfected modified MYOD1 mRNA to transdifferentiate human fibroblasts into myoblast-like cells without a transgene footprint. This efficient and integration-free mRNA technology opens the door for safe and controlled gene expression to reverse or redirect cell fate.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Cell Lineage
Cellular Reprogramming
Fibroblasts cytology
Gene Expression Profiling
Gene Expression Regulation
Humans
Interferons metabolism
MyoD Protein metabolism
Nucleosides metabolism
Transfection
Cell Transdifferentiation
Induced Pluripotent Stem Cells cytology
Myoblasts cytology
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 473
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 26449459
- Full Text :
- https://doi.org/10.1016/j.bbrc.2015.09.102