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Monitoring breast tumor progression by photoacoustic measurements: a xenograft mice model study.
- Source :
-
Journal of biomedical optics [J Biomed Opt] 2015 Oct; Vol. 20 (10), pp. 105002. - Publication Year :
- 2015
-
Abstract
- The current study reports the photoacoustic spectroscopy-based assessment of breast tumor progression in a nude mice xenograft model. The tumor was induced through subcutaneous injection of MCF-7 cells in female nude mice and was monitored for 20 days until the tumor volume reached 1000 mm3. The tumor tissues were extracted at three different time points (days 10, 15, and 20) after tumor inoculation and subjected to photoacoustic spectral recordings in time domain ex vivo at 281 nm pulsed laser excitations. The spectra were converted into the frequency domain using the fast Fourier transformed tools of MATLAB® algorithms and further utilized to extract seven statistical features (mean, median, area under the curve, variance and standard deviation, skewness and kurtosis) from each time point sample to assess the tumor growth with wavelet principal component analysis based logistic regression analysis performed on the data. The prediction accuracies of the analysis for day 10 versus day 15, day 15 versus day 20, and day 10 versus day 20 were found to be 92.31, 87.5, and 95.2%, respectively. Also, receiver operator characteristics area under the curve analysis for day 10 versus day 15, day 15 versus day 20, and day 10 versus day 20 were found to be 0.95, 0.85, and 0.93, respectively. The ability of photoacoustic measurements in the objective assessment of tumor progression has been clearly demonstrated, indicating its clinical potential.
- Subjects :
- Animals
Cell Line, Tumor
Equipment Design
Equipment Failure Analysis
Female
Humans
Image Enhancement methods
MCF-7 Cells
Mice
Mice, Nude
Neoplasm Invasiveness
Reproducibility of Results
Sensitivity and Specificity
Breast Neoplasms pathology
Image Interpretation, Computer-Assisted instrumentation
Image Interpretation, Computer-Assisted methods
Photoacoustic Techniques instrumentation
Photoacoustic Techniques methods
Subjects
Details
- Language :
- English
- ISSN :
- 1560-2281
- Volume :
- 20
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of biomedical optics
- Publication Type :
- Academic Journal
- Accession number :
- 26442962
- Full Text :
- https://doi.org/10.1117/1.JBO.20.10.105002