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The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi.
- Source :
-
Malaria journal [Malar J] 2015 Oct 05; Vol. 14, pp. 387. Date of Electronic Publication: 2015 Oct 05. - Publication Year :
- 2015
-
Abstract
- Background: Persistence of sulfadoxine-pyrimethamine (SP) resistance has been described in an urban setting in Malawi where malaria transmission is relatively low. Higher malaria transmission is associated with greater genetic diversity and more frequent genetic recombination, which could lead to a more rapid re-emergence of SP-sensitive parasites, as well as more rapid degradation of selective sweeps. In this study, the impact of local variation in malaria transmission on the prevalence of SP-resistant haplotypes and selective sweep characteristics was investigated at an urban site with low parasite prevalence and two rural sites with moderate and high parasite prevalence.<br />Methods: Samples from three sites with different parasite prevalence were genotyped for resistance markers within pfdhfr-ts and pfdhps and at microsatellites flanking these genes. Expected heterozygosity (He) was estimated to evaluate genetic diversity.<br />Results: No difference in the prevalence of highly resistant DHFR 51I/59R/108N and DHPS 437G/540E was found between sites. Small differences in He flanking pfdhfr-ts and pfdhps were seen between rural-moderate and the other sites, as well as some shared haplotypes between the rural-high and urban-low sites.<br />Conclusions: The results do not show an effect of local variation in malaria transmission, as inferred from parasite prevalence, on SP-resistant haplotype prevalence.
- Subjects :
- DNA, Protozoan genetics
Disease Transmission, Infectious
Drug Combinations
Genetic Variation
Genotype
Humans
Malaria epidemiology
Malawi epidemiology
Microsatellite Repeats
Peptide Synthases genetics
Plasmodium isolation & purification
Prevalence
Rural Population
Tetrahydrofolate Dehydrogenase genetics
Urban Population
Antimalarials pharmacology
Drug Resistance
Haplotypes
Malaria transmission
Plasmodium drug effects
Pyrimethamine pharmacology
Selection, Genetic
Sulfadoxine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2875
- Volume :
- 14
- Database :
- MEDLINE
- Journal :
- Malaria journal
- Publication Type :
- Academic Journal
- Accession number :
- 26437774
- Full Text :
- https://doi.org/10.1186/s12936-015-0860-7