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Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365.
- Source :
-
Chembiochem : a European journal of chemical biology [Chembiochem] 2015 Dec; Vol. 16 (18), pp. 2615-23. Date of Electronic Publication: 2015 Nov 06. - Publication Year :
- 2015
-
Abstract
- Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.<br /> (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Alanine analogs & derivatives
Alanine analysis
Amino Acid Sequence
Bacterial Proteins analysis
Bacterial Proteins isolation & purification
Chromatography, High Pressure Liquid
Molecular Sequence Data
Multigene Family
Peptides analysis
Peptides isolation & purification
Promoter Regions, Genetic
Protein Structure, Secondary
Sequence Alignment
Streptomyces genetics
Sulfides analysis
Tandem Mass Spectrometry
Bacterial Proteins biosynthesis
Peptides metabolism
Streptomyces metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1439-7633
- Volume :
- 16
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Chembiochem : a European journal of chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 26437689
- Full Text :
- https://doi.org/10.1002/cbic.201500377