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Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates.
- Source :
-
Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2016 Jun; Vol. 87 (6), pp. 809-15. Date of Electronic Publication: 2015 Oct 05. - Publication Year :
- 2016
-
Abstract
- Lactobacillus rhamnosus GG (LGG) is a well-established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme-linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance-based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β-galactoside at the non-reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β-galactoside at the non-reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions.<br /> (© 2015 Japanese Society of Animal Science.)
- Subjects :
- Animals
Bacterial Adhesion
Bacterial Proteins metabolism
Galactosides metabolism
Glycoconjugates chemistry
Glycoconjugates physiology
Intestinal Mucosa metabolism
Intestinal Mucosa microbiology
Membrane Proteins metabolism
Mice
Mucins chemistry
Oligosaccharides metabolism
Periodic Acid pharmacology
Probiotics
Protein Binding drug effects
Recombinant Proteins
Bacterial Proteins physiology
Fimbriae Proteins metabolism
Glycoconjugates metabolism
Lacticaseibacillus rhamnosus physiology
Membrane Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1740-0929
- Volume :
- 87
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Animal science journal = Nihon chikusan Gakkaiho
- Publication Type :
- Academic Journal
- Accession number :
- 26434750
- Full Text :
- https://doi.org/10.1111/asj.12491