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Addition of N-glycosylation sites on the globular head of the H5 hemagglutinin induces the escape of highly pathogenic avian influenza A H5N1 viruses from vaccine-induced immunity.
- Source :
-
Virology [Virology] 2015 Dec; Vol. 486, pp. 134-45. Date of Electronic Publication: 2015 Oct 01. - Publication Year :
- 2015
-
Abstract
- Highly pathogenic avian influenza A H5N1 viruses remain endemic in poultry in several countries and still constitute a pandemic threat. Since the early 20th century, we experienced four influenza A pandemics. H3N2 and H1N1pdm09 viruses that respectively emerged during 1968 and 2009 pandemics are still responsible for seasonal epidemics. These viruses evolve regularly by substitutions in antigenic sites of the hemagglutinin (HA), which prevent neutralization by antibodies directed against previous strains (antigenic drift). For seasonal H3N2 viruses, an addition of N-glycosylation sites (glycosites) on H3 contributed to this drift. Here, we questioned whether additional glycosites on H5 could induce an escape of H5N1 virus from neutralization, as it was observed for seasonal H3N2 viruses. Seven H5N1 mutants were produced by adding glycosites on H5. The most glycosylated virus escaped from neutralizing antibodies, in vitro and in vivo. Furthermore, a single additional glycosite was responsible for this escape.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Motifs
Animals
Antibodies, Neutralizing immunology
Antibodies, Viral immunology
Epitope Mapping
Female
Glycosylation
Hemagglutinin Glycoproteins, Influenza Virus chemistry
Hemagglutinin Glycoproteins, Influenza Virus genetics
Hemagglutinin Glycoproteins, Influenza Virus metabolism
Humans
Influenza A Virus, H3N2 Subtype chemistry
Influenza A Virus, H3N2 Subtype genetics
Influenza A Virus, H3N2 Subtype immunology
Influenza A Virus, H3N2 Subtype metabolism
Influenza A Virus, H5N1 Subtype chemistry
Influenza A Virus, H5N1 Subtype genetics
Influenza A Virus, H5N1 Subtype metabolism
Influenza Vaccines administration & dosage
Influenza Vaccines immunology
Influenza, Human virology
Mice
Mice, Inbred BALB C
Neutralization Tests
Hemagglutinin Glycoproteins, Influenza Virus immunology
Influenza A Virus, H5N1 Subtype immunology
Influenza, Human immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 486
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 26433051
- Full Text :
- https://doi.org/10.1016/j.virol.2015.08.033