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Association of BAFF, APRIL serum levels, BAFF-R, TACI and BCMA expression on peripheral B-cell subsets with clinical manifestations in systemic lupus erythematosus.
- Source :
-
Lupus [Lupus] 2016 May; Vol. 25 (6), pp. 582-92. Date of Electronic Publication: 2015 Sep 29. - Publication Year :
- 2016
-
Abstract
- Objective: B-cell-activating factor (BAFF) and a proliferation-inducing ligand (APRIL) signaling pathways regulate B-cell survival through interactions with their receptors BAFF-R, TACI and BCMA. We evaluated the association of these ligands/receptors on B-cell subsets according to clinical manifestations of systemic lupus erythematosus (SLE).<br />Methods: BAFF and APRIL serum concentrations were measured in 30 SLE patients by enzyme-linked immunosorbent assay. The BAFF-R, TACI and BCMA expression was analyzed on each B cell subset (CD19 + CD27-CD38-/ + naïve; CD19 + CD27 + CD38-/ + memory; CD19 + CD27-CD38 + + immature and CD19 + CD27 + CD38 + + plasma cells) by flow cytometry, and compared among patients with different clinical manifestations as well as healthy controls (HCs).<br />Results: Serum BAFF and APRIL levels were high in SLE patients and correlated with the Mex-SLEDAI disease activity index (r = 0.584; p = 0.001 and r = 0.456; p = 0.011, respectively). The SLE patients showed an increased proportion of memory and plasma B cells (p < 0.05). BAFF-R, TACI and BCMA expression in SLE patients was decreased in almost all B cell subsets compared to HCs (p < 0.05). A lower BCMA expression was associated with severe disease activity, glomerulonephritis, serositis and hemolytic anemia (p < 0.01). BCMA expression showed a negative correlation with Mex-SLEDAI score (r = -0.494, p = 0.006).<br />Conclusions: Decreased BCMA expression on peripheral B cells according to severe disease activity suggests that BCMA plays an important regulating role in B-cell hyperactivity and immune tolerance homeostasis in SLE patients.<br /> (© The Author(s) 2015.)
- Subjects :
- Adolescent
Adult
Aged
B-Cell Activation Factor Receptor genetics
B-Cell Maturation Antigen genetics
Case-Control Studies
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Humans
Immune Tolerance
Lupus Erythematosus, Systemic immunology
Middle Aged
Severity of Illness Index
Transmembrane Activator and CAML Interactor Protein genetics
Young Adult
B-Cell Activating Factor blood
B-Lymphocyte Subsets metabolism
Lupus Erythematosus, Systemic physiopathology
Tumor Necrosis Factor Ligand Superfamily Member 13 blood
Subjects
Details
- Language :
- English
- ISSN :
- 1477-0962
- Volume :
- 25
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Lupus
- Publication Type :
- Academic Journal
- Accession number :
- 26424128
- Full Text :
- https://doi.org/10.1177/0961203315608254