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Analgesic-antitumor peptide inhibits the migration and invasion of HepG2 cells by an upregulated VGSC β1 subunit.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2016 Mar; Vol. 37 (3), pp. 3033-41. Date of Electronic Publication: 2015 Sep 29. - Publication Year :
- 2016
-
Abstract
- Analgesic-antitumor peptide (AGAP), one of the scorpion toxin polypeptides, has been shown to have an antitumor activity. Recombinant AGAP (rAGAP) was shown to affect the migration and invasion of HepG2 cells via a voltage-gated sodium channel (VGSC) β1 subunit. The VGSC β1 subunit was validated as a cell adhesion molecule (CAM) in human hepatocellular carcinoma (HCC) cell lines. rAGAP suppresses the migration and invasion of HepG2 cells but has no significant effect of human liver HL7702 cells without β1 subunit expression. rAGAP inhibits the migration and invasion of the cells when the VGSC β1 subunit is overexpressed in HL7702 cells. To explain these findings, VGSC β1 subunit messenger RNA (mRNA) and protein levels were measured. The β1 subunit protein level was upregulated in a dose-dependent manner following treatment with rAGAP while there was no significant change in the mRNA level, so rAGAP might be an active component of the VGSC β1 subunit.
- Subjects :
- Cell Movement drug effects
Hep G2 Cells
Humans
Neoplasm Invasiveness
Recombinant Proteins pharmacology
Voltage-Gated Sodium Channel beta-1 Subunit analysis
Antineoplastic Agents pharmacology
Peptides pharmacology
Scorpion Venoms pharmacology
Voltage-Gated Sodium Channel beta-1 Subunit physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26419595
- Full Text :
- https://doi.org/10.1007/s13277-015-4067-x