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PepO, a CovRS-controlled endopeptidase, disrupts Streptococcus pyogenes quorum sensing.
- Source :
-
Molecular microbiology [Mol Microbiol] 2016 Jan; Vol. 99 (1), pp. 71-87. Date of Electronic Publication: 2015 Oct 14. - Publication Year :
- 2016
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Abstract
- Group A Streptococcus (GAS, Streptococcus pyogenes) is a human-restricted pathogen with a capacity to both colonize asymptomatically and cause illnesses ranging from pharyngitis to necrotizing fasciitis. An understanding of how and when GAS switches between genetic programs governing these different lifestyles has remained an enduring mystery and likely requires carefully tuned environmental sensors to activate and silence genetic schemes when appropriate. Herein, we describe the relationship between the Control of Virulence (CovRS, CsrRS) two-component system and the Rgg2/3 quorum-sensing pathway. We demonstrate that responses of CovRS to the stress signals Mg(2+) and a fragment of the antimicrobial peptide LL-37 result in modulated activity of pheromone signaling of the Rgg2/3 pathway through a means of proteolysis of SHP peptide pheromones. This degradation is mediated by the cytoplasmic endopeptidase PepO, which is the first identified enzymatic silencer of an RRNPP-type quorum-sensing pathway. These results suggest that under conditions in which the virulence potential of GAS is elevated (i.e. enhanced virulence gene expression), cellular responses mediated by the Rgg2/3 pathway are abrogated and allow individuals to escape from group behavior. These results also indicate that Rgg2/3 signaling is instead functional during non-virulent GAS lifestyles.<br /> (© 2015 John Wiley & Sons Ltd.)
- Subjects :
- Antimicrobial Cationic Peptides metabolism
Bacterial Proteins metabolism
Gene Regulatory Networks
Histidine Kinase
Intracellular Signaling Peptides and Proteins metabolism
Magnesium metabolism
Repressor Proteins metabolism
Streptococcus pyogenes drug effects
Streptococcus pyogenes pathogenicity
Virulence
Cathelicidins
Endopeptidases metabolism
Pheromones metabolism
Quorum Sensing
Signal Transduction
Streptococcus pyogenes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2958
- Volume :
- 99
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26418177
- Full Text :
- https://doi.org/10.1111/mmi.13216