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Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing.
- Source :
-
PloS one [PLoS One] 2015 Sep 29; Vol. 10 (9), pp. e0139274. Date of Electronic Publication: 2015 Sep 29 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Zinc finger protein 809 (ZFP809) is a member of the Kruppel-associated box-containing zinc finger protein (KRAB-ZFP) family, and is highly expressed in mouse immature cells. ZFP809 is known to inhibit the expression of transduced genes driven by Moloney murine leukemia virus (MoMLV)-typed retroviral vectors by binding to the primer binding site (PBS) located downstream of the MLV-long terminal repeat (LTR) of the vectors and recruiting protein complexes that introduce epigenetic silencing marks such as histone modifications and DNA methylation at the MLV-LTR. However, it remains undetermined what domains of ZFP809 among the KRAB domain at N-terminus and the seven zinc fingers are critical for gene silencing. In this study, we assessed subcellular localization, gene silencing ability, and binding ability to the PBS of a series of truncated and mutated ZFP809 proteins. We revealed the essential role of the KRAB A box for all functions assessed, together with the accessory roles of a subset of zinc fingers. Our data also suggest that interaction between KAP1 and the KRAB A box of ZFP809 is critical in KAP1-dependent control of gene silencing for ZFP809 targets.
- Subjects :
- Animals
Binding Sites genetics
Blotting, Western
DNA-Binding Proteins metabolism
HEK293 Cells
Humans
Luminescent Proteins genetics
Luminescent Proteins metabolism
Mice
Microscopy, Fluorescence
Models, Genetic
Moloney murine leukemia virus genetics
Mutation
Nuclear Proteins metabolism
Protein Binding
Repressor Proteins genetics
Repressor Proteins metabolism
Terminal Repeat Sequences genetics
Tripartite Motif-Containing Protein 28
Cell Nucleus metabolism
DNA-Binding Proteins genetics
Gene Silencing
Nuclear Proteins genetics
Zinc Fingers genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26417948
- Full Text :
- https://doi.org/10.1371/journal.pone.0139274