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Astaxanthin Inhibits Proliferation and Induces Apoptosis of Human Hepatocellular Carcinoma Cells via Inhibition of Nf-Κb P65 and Wnt/Β-Catenin in Vitro.
- Source :
-
Marine drugs [Mar Drugs] 2015 Sep 24; Vol. 13 (10), pp. 6064-81. Date of Electronic Publication: 2015 Sep 24. - Publication Year :
- 2015
-
Abstract
- Hepatocellular carcinoma (HCC) is a malignant tumor that can cause systemic invasion; however, the exact etiology and molecular mechanism are unknown. Astaxanthin (ASX), a powerful antioxidant, has efficient anti-oxidant, anti-inflammatory, and other activities, and has great research prospects in cancer therapy. We selected the human hepatoma cell lines, LM3 and SMMC-7721, to study the anti-tumor effect and related mechanisms of ASX. The cell lines were treated with different concentrations of ASX, and its solvent DMSO as a control, for different time periods and the results were determined using CCK8, qRT-PCR, WB, apoptotic staining, and flow cytometry. ASX induced significant apoptosis of HCC cells, and its effect may have been caused by NF-κB p65 and Wnt/β-catenin down-regulation via negative activation of PI3K/Akt and ERK. Antitumor research on ASX has provided us with a potential therapy for patients with hepatomas.
- Subjects :
- Antineoplastic Agents administration & dosage
Apoptosis drug effects
Carcinoma, Hepatocellular pathology
Cell Line, Tumor
Dose-Response Relationship, Drug
Down-Regulation drug effects
Flow Cytometry
Humans
Liver Neoplasms pathology
Transcription Factor RelA genetics
Wnt Proteins genetics
Xanthophylls administration & dosage
Xanthophylls pharmacology
beta Catenin genetics
Antineoplastic Agents pharmacology
Carcinoma, Hepatocellular drug therapy
Liver Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 13
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 26404320
- Full Text :
- https://doi.org/10.3390/md13106064