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Alt a 15 is a new cross-reactive minor allergen of Alternaria alternata.

Authors :
Gabriel MF
Postigo I
Gutiérrez-Rodríguez A
Suñén E
Guisantes JA
Fernández J
Tomaz CT
Martínez J
Source :
Immunobiology [Immunobiology] 2016 Feb; Vol. 221 (2), pp. 153-60. Date of Electronic Publication: 2015 Sep 10.
Publication Year :
2016

Abstract

Alternaria alternata is one of the most common saprophytes worldwide that is clinically and epidemiologically associated with severe asthma. Therefore, the identification and characterization of all A. alternata allergens are of major clinical importance. This study describes a new cross-reactive A. alternata allergen that was officially named Alt a 15 by the official Allergen Nomenclature Subcommittee. The complete coding region for Alt a 15 was amplified using 5' and 3' rapid amplification of cDNA ends and PCR. The recombinant protein was produced in Escherichia coli as a 65-kDa fusion protein, and the protein sequence exhibits high homology with several important fungal allergens. Immunoblotting analyses revealed that IgE antibodies from A. alternata-sensitized patients (n=59) bound to rAlt a 15 with a prevalence of 10.2%. All patients who presented sIgE to rAlt a 15 were apparently poly-sensitized to A. alternata and C. lunata. The extensive cross-reactivity between A. alternata and C. lunata serine proteases was confirmed using immunoblotting inhibition assays. Overall, Alt a 15 is an important new cross-reactive allergen of A. alternata that explains some allergies to A. alternata without Alt a 1 sensitization and initial diagnostic errors for allergies to Alternaria. This molecule may improve the accuracy of the diagnosis, the understanding, and the management of IgE-mediated fungal diseases.<br /> (Copyright © 2015 Elsevier GmbH. All rights reserved.)

Details

Language :
English
ISSN :
1878-3279
Volume :
221
Issue :
2
Database :
MEDLINE
Journal :
Immunobiology
Publication Type :
Academic Journal
Accession number :
26395961
Full Text :
https://doi.org/10.1016/j.imbio.2015.09.014