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Alt a 15 is a new cross-reactive minor allergen of Alternaria alternata.
- Source :
-
Immunobiology [Immunobiology] 2016 Feb; Vol. 221 (2), pp. 153-60. Date of Electronic Publication: 2015 Sep 10. - Publication Year :
- 2016
-
Abstract
- Alternaria alternata is one of the most common saprophytes worldwide that is clinically and epidemiologically associated with severe asthma. Therefore, the identification and characterization of all A. alternata allergens are of major clinical importance. This study describes a new cross-reactive A. alternata allergen that was officially named Alt a 15 by the official Allergen Nomenclature Subcommittee. The complete coding region for Alt a 15 was amplified using 5' and 3' rapid amplification of cDNA ends and PCR. The recombinant protein was produced in Escherichia coli as a 65-kDa fusion protein, and the protein sequence exhibits high homology with several important fungal allergens. Immunoblotting analyses revealed that IgE antibodies from A. alternata-sensitized patients (n=59) bound to rAlt a 15 with a prevalence of 10.2%. All patients who presented sIgE to rAlt a 15 were apparently poly-sensitized to A. alternata and C. lunata. The extensive cross-reactivity between A. alternata and C. lunata serine proteases was confirmed using immunoblotting inhibition assays. Overall, Alt a 15 is an important new cross-reactive allergen of A. alternata that explains some allergies to A. alternata without Alt a 1 sensitization and initial diagnostic errors for allergies to Alternaria. This molecule may improve the accuracy of the diagnosis, the understanding, and the management of IgE-mediated fungal diseases.<br /> (Copyright © 2015 Elsevier GmbH. All rights reserved.)
- Subjects :
- Allergens chemistry
Allergens genetics
Alternaria chemistry
Amino Acid Sequence
Antibodies, Fungal isolation & purification
Antibody Specificity
Antigens, Fungal chemistry
Antigens, Fungal genetics
Asthma chemically induced
Asthma genetics
Asthma microbiology
Cloning, Molecular
Cross Reactions
Escherichia coli genetics
Escherichia coli metabolism
Gene Expression
Humans
Immunoglobulin E isolation & purification
Molecular Sequence Data
Open Reading Frames
Protein Binding
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins immunology
Sequence Alignment
Allergens immunology
Alternaria immunology
Antibodies, Fungal chemistry
Antigens, Fungal immunology
Asthma immunology
Immunoglobulin E chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3279
- Volume :
- 221
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 26395961
- Full Text :
- https://doi.org/10.1016/j.imbio.2015.09.014