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Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells.
- Source :
-
PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2015 Sep 22; Vol. 9 (9), pp. e0003958. Date of Electronic Publication: 2015 Sep 22 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Dengue viruses (DENVs) are among the most rapidly and efficiently spreading arboviruses. WHO recently estimated that about half of the world's population is now at risk for DENV infection. There is no specific treatment or vaccine available to treat or prevent DENV infections. Here, we report the development of a novel dengue nanovaccine (DNV) composed of UV-inactivated DENV-2 (UVI-DENV) and Mycobacterium bovis Bacillus Calmette-Guerin cell wall components (BCG-CWCs) loaded into chitosan nanoparticles (CS-NPs). CS-NPs were prepared by an emulsion polymerization method prior to loading of the BCG-CWCs and UVI-DENV components. Using a scanning electron microscope and a zetasizer, DNV was determined to be of spherical shape with a diameter of 372.0 ± 11.2 nm in average and cationic surface properties. The loading efficacies of BCG-CWCs and UVI-DENV into the CS-NPs and BCG-CS-NPs were up to 97.2 and 98.4%, respectively. THP-1 cellular uptake of UVI-DENV present in the DNV was higher than soluble UVI-DENV alone. DNV stimulation of immature dendritic cells (iDCs) resulted in a significantly higher expression of DCs maturation markers (CD80, CD86 and HLA-DR) and induction of various cytokine and chemokine productions than in UVI-DENV-treated iDCs, suggesting a potential use of BCG- CS-NPs as adjuvant and delivery system for dengue vaccines.
- Subjects :
- Adjuvants, Immunologic metabolism
B7-1 Antigen analysis
B7-2 Antigen analysis
Cell Differentiation drug effects
Cytokines metabolism
Dengue Vaccines metabolism
Endocytosis
HLA-DR Antigens analysis
Humans
Immunophenotyping
Microscopy, Electron, Scanning
Monocytes metabolism
Nanoparticles ultrastructure
Vaccines, Inactivated immunology
Vaccines, Inactivated metabolism
Antigens, Bacterial metabolism
Chitosan metabolism
Dendritic Cells immunology
Dengue Vaccines immunology
Dengue Virus immunology
Mycobacterium bovis chemistry
Nanoparticles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1935-2735
- Volume :
- 9
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PLoS neglected tropical diseases
- Publication Type :
- Academic Journal
- Accession number :
- 26394138
- Full Text :
- https://doi.org/10.1371/journal.pntd.0003958