A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS.

Authors :: Adams LL
Salee P
Dionne K
Carroll K
Parrish N
Source :: Journal of microbiological methods [J Microbiol Methods] 2015 Dec; Vol. 119, pp. 1-3. Date of Electronic Publication: 2015 Sep 25.
Publication Year :: 2015
Abstract: Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Humans
Mycobacterium genetics
Mycobacterium growth & development
Mycobacterium isolation & purification
Solid Phase Extraction instrumentation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Mycobacterium chemistry
Mycobacterium Infections microbiology
Solid Phase Extraction methods

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