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Synergistic effects of rhubarb-gardenia herb pair in cholestatic rats at pharmacodynamic and pharmacokinetic levels.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2015 Dec 04; Vol. 175, pp. 67-74. Date of Electronic Publication: 2015 Sep 13. - Publication Year :
- 2015
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Abstract
- Ethnopharmacological Relevance: Herb pair serves as the basic building block of a traditional Chinese medicine (TCM) formula. The rhubarb-gardenia herb pair (RGHP), composed of rhubarb and gardenia, has meaningful clinical effects to cure cholestasis diseases. This study was designed to confirm the expected synergistic effects of RGHP at pharmacodynamic and pharmacokinetic levels.<br />Materials and Methods: Thirty male Sprague-Dawley rats were divided into control, model and drug-treated groups. After intragastrically administrated with α-naphthylisothiocyanate (ANIT) to induce cholestasis, rats were treated with rhubarb, gardenia or RGHP. For pharmacodynamic study, biochemical and histopathological tests were performed to assess the hepatoprotective effects. While for pharmacokinetic study, a LC-MS method was developed for determination of five main chemical markers, namely genipin, rhein, aloe emodin, emodin and chrysophanol in rat plasma.<br />Results: The biochemical and histopathological tests suggested that RGHP exerted enhanced hepatoprotective effects against the ANIT-induced cholestasis compared with single herbs. The pharmacokinetic study indicated RGHP could significantly elevate systemic exposure level and prolong retention time of five markers in comparison with rhubarb or gardenia alone.<br />Conclusions: The present study demonstrated the synergistic effects of RGHP in ANIT-induced cholestatic rats at pharmacodynamic and pharmacokinetic levels, and has significant enlightenments for the rational use of the related TCM formulas containing RGHP.<br /> (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- 1-Naphthylisothiocyanate
Alanine Transaminase blood
Alkaline Phosphatase blood
Animals
Anthraquinones blood
Aspartate Aminotransferases blood
Bilirubin blood
Drug Synergism
Emodin blood
Fruit
Iridoids blood
Liver drug effects
Liver pathology
Male
Rats, Sprague-Dawley
Rhizome
Cholestasis chemically induced
Cholestasis metabolism
Cholestasis pathology
Gardenia
Plant Extracts blood
Plant Extracts pharmacokinetics
Plant Extracts pharmacology
Protective Agents pharmacokinetics
Protective Agents pharmacology
Rheum
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 175
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26376237
- Full Text :
- https://doi.org/10.1016/j.jep.2015.09.012