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Experimental evaluation of the zoonotic infection potency of simian retrovirus type 4 using humanized mouse model.
- Source :
-
Scientific reports [Sci Rep] 2015 Sep 14; Vol. 5, pp. 14040. Date of Electronic Publication: 2015 Sep 14. - Publication Year :
- 2015
-
Abstract
- During 2001-2002 and 2008-2011, two epidemic outbreaks of infectious hemorrhagic disease have been found in Japanese macaques (Macaca fuscata) in Kyoto University Primate Research Institute, Japan. Following investigations revealed that the causative agent was simian retrovirus type 4 (SRV-4). SRV-4 was isolated by using human cell lines, which indicates that human cells are potently susceptible to SRV-4 infection. These raise a possibility of zoonotic infection of pathogenic SRV-4 from Japanese macaques into humans. To explore the possibility of zoonotic infection of SRV-4 to humans, here we use a human hematopoietic stem cell-transplanted humanized mouse model. Eight out of the twelve SRV-4-inoculated humanized mice were infected with SRV-4. Importantly, 3 out of the 8 infected mice exhibited anemia and hemophagocytosis, and an infected mouse died. To address the possibility that SRV-4 adapts humanized mouse and acquires higher pathogenicity, the virus was isolated from an infected mice exhibited severe anemia was further inoculated into another 6 humanized mice. However, no infected mice exhibited any illness. Taken together, our findings demonstrate that the zoonotic SRV-4 infection from Japanese macaques to humans is technically possible under experimental condition. However, such zoonotic infection may not occur in the real society.
- Subjects :
- APOBEC Deaminases
Animals
Cell Line
Cytidine Deaminase
Cytokines metabolism
Cytosine Deaminase metabolism
Female
HEK293 Cells
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells metabolism
Hematopoietic Stem Cells virology
Humans
Japan
Male
Mice
Mice, Inbred NOD
Mice, Transgenic
Models, Animal
Polymerase Chain Reaction
RNA, Viral analysis
Retroviridae Infections pathology
Retroviridae Infections virology
Retroviruses, Simian genetics
Retroviruses, Simian isolation & purification
Transplantation, Heterologous
Zoonoses virology
Retroviridae Infections transmission
Retroviruses, Simian pathogenicity
Zoonoses transmission
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26364986
- Full Text :
- https://doi.org/10.1038/srep14040