Back to Search
Start Over
Effects of acute ethanol exposure on class I HDACs family enzymes in wild-type and BDNF(+/-) mice.
- Source :
-
Drug and alcohol dependence [Drug Alcohol Depend] 2015 Oct 01; Vol. 155, pp. 68-75. Date of Electronic Publication: 2015 Aug 28. - Publication Year :
- 2015
-
Abstract
- Background: Alterations of brain-derived neurotrophic factor (BDNF) have been associated with the development of addiction to different drugs of abuse, including ethanol (EtOH). EtOH exposure activates the BDNF-signaling cascade in dorsal striatum, which in turn affects further EtOH intake. Different alcohol exposures have been widely demonstrated to modulate chromatin remodeling, affecting histone acetylation/deacetylation balance. Recently, class I histone deacetylases (HDACs) inhibition has been reported to modulate BDNF mRNA expression and to attenuate morphological and behavioral phenomena related to EtOH exposure. However, the role played by different HDAC isoforms in EtOH-induced plasticity is still unclear.<br />Methods: We investigated the effects induced by acute EtOH exposure on the protein levels of class I HDAC 1-3 isoforms of wild-type (WT) and BDNF heterozygous mice (BDNF(+/-)), in nuclear and cytoplasmic extracts of specific brain regions associated with EtOH addiction.<br />Results: Nuclear HDAC 1-3 levels were markedly reduced after acute EtOH treatment in the caudate putamen (CPu) of WT mice only. Furthermore, CPu basal levels of nuclear HDAC isoforms were significantly lower in BDNF(+/-) mice compared to WT. With the exception of nuclear HDAC 3, no significant changes were observed after acute EtOH treatment in the prefrontal cortex (PFCx) of BDNF(+/-) and WT mice. In this area, the nuclear HDAC basal levels were significantly different between the two experimental groups.<br />Conclusions: These results provide details about EtOH effects on class I HDAC isoforms and strongly support a correlation between BDNF and class I HDACs, suggesting a possible influence of BNDF on these enzymes.<br /> (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Nucleus drug effects
Cell Nucleus enzymology
Cytoplasm drug effects
Cytoplasm enzymology
Female
Heterozygote
Isoenzymes metabolism
Male
Mice
Prefrontal Cortex drug effects
Prefrontal Cortex enzymology
Putamen drug effects
Putamen enzymology
Rotarod Performance Test
Brain-Derived Neurotrophic Factor genetics
Ethanol administration & dosage
Ethanol pharmacology
Histone Deacetylases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0046
- Volume :
- 155
- Database :
- MEDLINE
- Journal :
- Drug and alcohol dependence
- Publication Type :
- Academic Journal
- Accession number :
- 26361715
- Full Text :
- https://doi.org/10.1016/j.drugalcdep.2015.08.015