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Methods in HPV Surveillance: Experiences from a Population-Based Study of HPV Infection among Women in the San Juan Metropolitan Area of Puerto Rico.
- Source :
-
Puerto Rico health sciences journal [P R Health Sci J] 2015 Sep; Vol. 34 (3), pp. 117-27. - Publication Year :
- 2015
-
Abstract
- This article describes the methodology of the first population-based study of human papillomavirus (HPV) infection among women aged 16-64 years residing in the San Juan Metropolitan Area of Puerto Rico (PR). The sample was identified through a complex sampling design of households. The sampling frame was selected in four stages, using census tracts maps from the Census Bureau. Women completed a face-to-face interview and a computer-assisted self-interview using the Audio CASI system, for the collection of demographic, clinical, and lifestyle variables, and sampling acceptability. Anal, cervical, and oral specimens were collected through self-collection methods for HPV DNA testing using a modified pool of MY09/MY11 consensus HPV L1 and human ß-globin amplification primers. Anthropometric measurements were taken using the Third National Health and Nutrition Examination Survey methodology. Blood samples were collected to create a bio-repository for future HPV-related studies. Fifty census tract blocks were randomly selected. We recruited 566 women, with a response rate of 83.4%. Response rates did not vary by age-group (p>0.05); although they varied by socioeconomic (SES) census block stratums (p<0.05), response rates were good (>75%) in all SES strata. All participants agreed to respond to the surveys and provide the requested anogenital and oral samples. Overall, more than 98% understood and more than 50% felt comfortable with the cervical, anal, and oral self-collection methods used. This article documents the feasibility of performing population-based studies for HPV surveillance in women in PR.
- Subjects :
- Adolescent
Adult
Feasibility Studies
Female
Humans
Middle Aged
Nutrition Surveys
Papillomavirus Infections diagnosis
Papillomavirus Infections virology
Population Surveillance methods
Puerto Rico epidemiology
Young Adult
DNA, Viral isolation & purification
Papillomaviridae isolation & purification
Papillomavirus Infections epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 0738-0658
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Puerto Rico health sciences journal
- Publication Type :
- Academic Journal
- Accession number :
- 26356735