Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles.

Authors :: Jaé N
McEwan DG
Manavski Y
Boon RA
Dimmeler S
Source :: FEBS letters [FEBS Lett] 2015 Oct 07; Vol. 589 (20 Pt B), pp. 3182-8. Date of Electronic Publication: 2015 Sep 05.
Publication Year :: 2015
Abstract: By transporting regulatory RNAs like microRNAs, extracellular vesicles provide a novel layer of intercellular gene regulation. However, the underlying secretory pathways and the mechanisms of cargo selection are poorly understood. Rab GTPases are central coordinators of membrane trafficking with distinct members of this family being responsible for specific transport pathways. Here we identified a vesicular export mechanism for miR-143, induced by the shear stress responsive transcription factor KLF2, and demonstrate its dependency on Rab7a/Rab27b in endothelial cells.<br /> (Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

Subjects :: Blotting, Western
Cells, Cultured
Extracellular Vesicles genetics
Gene Expression
HeLa Cells
Humans
Kruppel-Like Transcription Factors genetics
Kruppel-Like Transcription Factors metabolism
MicroRNAs genetics
Microscopy, Confocal
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Stress, Mechanical
rab GTP-Binding Proteins genetics
rab7 GTP-Binding Proteins
Extracellular Vesicles metabolism
Human Umbilical Vein Endothelial Cells metabolism
MicroRNAs metabolism
rab GTP-Binding Proteins metabolism

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