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Identification of human ELOVL5 enhancer regions controlled by SREBP.

Authors :
Shikama A
Shinozaki H
Takeuchi Y
Matsuzaka T
Aita Y
Murayama T
Sawada Y
Piao X
Toya N
Oya Y
Takarada A
Masuda Y
Nishi M
Kubota M
Izumida Y
Nakagawa Y
Iwasaki H
Kobayashi K
Yatoh S
Suzuki H
Yagyu H
Kawakami Y
Yamada N
Shimano H
Yahagi N
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 Oct 02; Vol. 465 (4), pp. 857-63. Date of Electronic Publication: 2015 Aug 28.
Publication Year :
2015

Abstract

Fatty acid elongase 5 (ELOVL5) is an enzyme involved in the synthesis of polyunsaturated fatty acids. Sterol Regulatory Element-binding Protein (SREBP)-1 activates ELOVL5 and increases polyunsaturated fatty acid synthesis, which in turn negatively affects SREBP-1 expression. Thus, ELOVL5 has been established as an SREBP-1 target gene and an important component of the negative feedback loop of de novo lipogenesis. However, the human ELOVL5 promoter/enhancer has not been fully analyzed and the location of SREBP biding sites around the ELOVL5 gene has yet to be defined. Here we performed a detailed promoter/enhancer analysis of human ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1. These two SRE motifs are conserved among mammals and the mechanism found in the present study by which SREBP activates ELOVL5 is considered to be common in mammals. Through these findings, we clarified the molecular mechanism how SREBP activates ELOVL5, an important regulator of de novo lipogenesis.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1090-2104
Volume :
465
Issue :
4
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
26321664
Full Text :
https://doi.org/10.1016/j.bbrc.2015.08.101