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Cytotoxic Screening and In Vitro Evaluation of Pentadecane Against Leishmania infantum Promastigotes and Amastigotes.
- Source :
-
The Journal of parasitology [J Parasitol] 2015 Dec; Vol. 101 (6), pp. 701-5. Date of Electronic Publication: 2015 Aug 31. - Publication Year :
- 2015
-
Abstract
- Pentadecane is an organic compound that is made up of primarily carbon and hydrogen atoms. Pentadecane is a floral volatile found in many different plants and essential oils and also in crude extracts of some plants, and it shows antimicrobial activity. This study investigated in vitro effects of pentadecane in Leishmania infantum parasites and found that it decreases growth by 86 ± 2% both in promastigotes (half maximal inhibitory concentration [IC50] = 65.3 μM) and in amastigotes (IC50 = 60.5 μM), resulting in a reduction of macrophage infection; growth inhibition was 77% at 300 μM. Analysis of propidium iodide incorporation in L. infantum , treated with pentadecane at 48 hr, suggested that cells were arresting in the sub-G0/G1 and G1 phases of the cell cycle, whereas cytotoxicity assay of pentadecane in immortalized cells lines DH82 and U937 and in primary epithelial cells of Cercopiteco showed that it caused negligible cytotoxic effect. This study shows that pentadecane has antimicrobial activity against L. infantum parasites in in vitro culture.
- Subjects :
- Alkanes toxicity
Animals
Cell Line
Cercopithecus
Epithelial Cells drug effects
Flowers chemistry
G1 Phase drug effects
Humans
Inhibitory Concentration 50
Leishmania infantum cytology
Leishmania infantum growth & development
Macrophages drug effects
Macrophages parasitology
Plant Extracts toxicity
Resting Phase, Cell Cycle drug effects
U937 Cells
Alkanes pharmacology
Leishmania infantum drug effects
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1937-2345
- Volume :
- 101
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 26321352
- Full Text :
- https://doi.org/10.1645/15-736