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Innate and Adaptive Humoral Responses Coat Distinct Commensal Bacteria with Immunoglobulin A.
- Source :
-
Immunity [Immunity] 2015 Sep 15; Vol. 43 (3), pp. 541-53. Date of Electronic Publication: 2015 Aug 25. - Publication Year :
- 2015
-
Abstract
- Immunoglobulin A (IgA) is prominently secreted at mucosal surfaces and coats a fraction of the intestinal microbiota. However, the commensal bacteria bound by IgA are poorly characterized and the type of humoral immunity they elicit remains elusive. We used bacterial flow cytometry coupled with 16S rRNA gene sequencing (IgA-Seq) in murine models of immunodeficiency to identify IgA-bound bacteria and elucidate mechanisms of commensal IgA targeting. We found that residence in the small intestine, rather than bacterial identity, dictated induction of specific IgA. Most commensals elicited strong T-independent (TI) responses that originated from the orphan B1b lineage and from B2 cells, but excluded natural antibacterial B1a specificities. Atypical commensals including segmented filamentous bacteria and Mucispirillum evaded TI responses but elicited T-dependent IgA. These data demonstrate exquisite targeting of distinct commensal bacteria by multiple layers of humoral immunity and reveal a specialized function of the B1b lineage in TI mucosal IgA responses.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Adaptive Immunity genetics
Animals
B-Lymphocytes immunology
B-Lymphocytes metabolism
Bacteria classification
Bacteria genetics
Colon immunology
Colon metabolism
Colon microbiology
Flow Cytometry
Genetic Variation immunology
Humans
Immunity, Humoral genetics
Immunity, Innate genetics
Immunoglobulin A metabolism
Intestine, Small metabolism
Intestine, Small microbiology
Mice, Inbred C57BL
Mice, Knockout
RNA, Ribosomal, 16S genetics
T-Lymphocytes immunology
T-Lymphocytes metabolism
Adaptive Immunity immunology
Bacteria immunology
Immunity, Humoral immunology
Immunity, Innate immunology
Immunoglobulin A immunology
Intestine, Small immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4180
- Volume :
- 43
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 26320660
- Full Text :
- https://doi.org/10.1016/j.immuni.2015.08.007