MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon.

Authors :: Duan X
Li H
Qiu Z
Joshi BP
Pant A
Smith A
Kurabayashi K
Oldham KR
Wang TD
Source :: Biomedical optics express [Biomed Opt Express] 2015 Jul 29; Vol. 6 (8), pp. 3074-83. Date of Electronic Publication: 2015 Jul 29 (Print Publication: 2015).
Publication Year :: 2015
Abstract: We demonstrate a handheld multiphoton endomicroscope with 3.4 mm distal diameter that can repetitively image mouse colon in vivo. A 2D resonant MEMS mirror was developed to perform beam scanning in a Lissajous pattern. The instrument has an effective numerical aperture of 0.63, lateral and axial resolution of 2.03 and 9.02 μm, respectively, working distance of 60 μm, and image field-of-view of 300 × 300 μm(2). Hoechst was injected intravenously in mice to stain cell nuclei. We were able to collect histology-like images in vivo at 5 frames/sec, and distinguish between normal and pre-malignant colonic epithelium.