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Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Based Electrospun 3D Scaffolds for Delivery of Autogeneic Chondrocytes and Adipose-Derived Stem Cells: Evaluation of Cartilage Defects in Rabbit.
- Source :
-
Journal of biomedical nanotechnology [J Biomed Nanotechnol] 2015 Jan; Vol. 11 (1), pp. 105-16. - Publication Year :
- 2015
-
Abstract
- The management of chondral defects has long been a challenge because of the poor self-healing capacity of articular cartilage. Many approaches ranging from symptomatic treatment to structural cartilage regeneration have obtained very limited satisfactory results. Cartilage tissue engineering, which involves an optimized combination of novel scaffolds, cell sources and growth factors, has emerged as a promising strategy for cartilage regeneration and repair. In this study, the cellular morphologies and the adhesion, migration and proliferation capabilities of adipose-derived stem cells (ASCs) and chondrocytes seeded on 3D scaffolds composed of electrospun poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3HB4HB) were evaluated. Next, TGF-β1/scaffolds with 4:1 co-culture of ASCs and chondrocytes were implanted into the full thickness cartilage defects in rabbit knee for 16 weeks. ASCs and chondrocytes seeded on the scaffolds showed better adhesion, migration and proliferation than that on petri dishes in vitro. Importantly, implantation with TGF-β1/scaffolds with delivery of ASCs and chondrocytes revealed desirable in vivo healing outcomes. These results demonstrate that ASCs have great potential in the field of tissue engineering. It is possible that the improvement in ASC-seeded electrospun 3D P3HB4HB scaffolds may ultimately lead to improved repair of cartilage injuries.
- Subjects :
- Adipocytes transplantation
Animals
Cells, Cultured
Chondrocytes cytology
Electroplating methods
Equipment Design
Equipment Failure Analysis
Fractures, Cartilage pathology
Rabbits
Rotation
Tissue Engineering instrumentation
Treatment Outcome
Adipocytes cytology
Chondrocytes transplantation
Fractures, Cartilage therapy
Polyesters chemistry
Stem Cell Transplantation methods
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1550-7033
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biomedical nanotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 26301304
- Full Text :
- https://doi.org/10.1166/jbn.2015.2053