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Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition.
- Source :
-
Scientific reports [Sci Rep] 2015 Aug 24; Vol. 5, pp. 13434. Date of Electronic Publication: 2015 Aug 24. - Publication Year :
- 2015
-
Abstract
- Schistosomiasis is a major human parasitic disease with a global impact. Schistosoma japonicum, the most difficult to control, can survive within host veins for decades. Mechanisms of immune evasion by the parasite, including antigenic variation and surface masking, have been implicated but not well defined. In this study, we defined the immunoglobulin-binding proteomes of S. japonicum using human IgG, IgM, and IgE as the molecular bait for affinity purification, followed by protein identification by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Several proteins situated at the tegument of S. japonicum were able to nonselectively bind to the Fc domain of host immunoglobulins, indicating a mechanism for the avoidance of host immune attachment and recognition. The profile of the immunoglobulin-binding proteomes provides further clues for immune evasion mechanisms adopted by S. japonicum.
- Subjects :
- Animals
Binding, Competitive
Cell Membrane metabolism
Cluster Analysis
Complement C1q metabolism
Female
Gene Expression Regulation
Humans
Life Cycle Stages genetics
Male
Mice, Inbred BALB C
Microscopy, Confocal
Parasites genetics
Parasites growth & development
Protein Binding
Proteomics
Protozoan Proteins metabolism
Rabbits
Receptors, Fc metabolism
Reproducibility of Results
Schistosoma japonicum genetics
Host-Parasite Interactions immunology
Immunoglobulins immunology
Schistosoma japonicum immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26299686
- Full Text :
- https://doi.org/10.1038/srep13434