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Caffeic acid phenethyl ester inhibits 3-MC-induced CYP1A1 expression through induction of hypoxia-inducible factor-1α.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 Sep 25; Vol. 465 (3), pp. 562-8. Date of Electronic Publication: 2015 Aug 18. - Publication Year :
- 2015
-
Abstract
- Caffeic acid phenethyl ester (CAPE), a natural component of propolis, is reported to have anticarcinogenic properties, although its precise chemopreventive mechanism remains unclear. In this study, we examined the effects of CAPE on 3-methylcholanthrene (3-MC)-induced CYP1A1 expression and activities. CAPE reduced the formation of the benzo[a]pyrene-DNA adduct. Moreover, CAPE inhibited 3-MC-induced CYP1A1 activity, mRNA expression, protein level, and promoter activity. CAPE treatment also decreased 3-MC-inducible xenobiotic-response element (XRE)-linked luciferase, aryl hydrocarbons receptor (AhR) transactivation and nuclear localization. CAPE induced hypoxia inducible factor-1α (HIF-1α) protein level and HIF-1α responsible element (HRE) transcriptional activity. CAPE-mediated HIF-1α reduced 3-MC-inducible CYP1A1 protein expression. Taken together, CAPE decreases 3-MC-mediated CYP1A1 expression, and this inhibitory response is associated with inhibition of AhR and HIF-1α induction.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Apoptosis drug effects
Cell Line, Tumor
Cell Survival drug effects
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic drug effects
Mice
Phenylethyl Alcohol administration & dosage
Caffeic Acids administration & dosage
Carcinoma, Hepatocellular drug therapy
Carcinoma, Hepatocellular metabolism
Cytochrome P-450 CYP1A1 metabolism
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Methylcholanthrene administration & dosage
Phenylethyl Alcohol analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 465
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 26296470
- Full Text :
- https://doi.org/10.1016/j.bbrc.2015.08.060