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Dog erythrocyte antigen 1: mode of inheritance and initial characterization.
- Source :
-
Veterinary clinical pathology [Vet Clin Pathol] 2015 Sep; Vol. 44 (3), pp. 369-79. Date of Electronic Publication: 2015 Aug 20. - Publication Year :
- 2015
-
Abstract
- Background: The dog erythrocyte antigen (DEA) 1 blood group system remains poorly defined.<br />Objectives: The purpose of the study was to determine the DEA 1 mode of inheritance and to characterize the DEA 1 antigen and alloantibodies.<br />Animals: Canine research colony families, clinic canine patients, and DEA 1.2+ blood bank dogs were studied.<br />Methods: Canine blood was typed by flow cytometry and immunochromatographic strips using anti-DEA 1 monoclonal antibodies. Gel column experiments with polyclonal and immunoblotting with monoclonal anti-DEA 1 antibodies were performed to analyze select samples. Cross-reactivity of human typing reagents against canine RBC and one monoclonal anti-DEA 1 antibody against human RBC panels was assessed.<br />Results: Typing of 12 families comprising 144 dogs indicated an autosomal dominant inheritance with ≥ 4 alleles: DEA 1- (0) and DEA 1+ weak (1+), intermediate (2+), and strong (3+ and 4+). Samples from 6 dogs previously typed as DEA 1.2+ were typed as DEA 1+ or DEA 1- using monoclonal antibodies. Human typing reagents produced varied reactions in tube agglutination experiments against DEA 1+ and DEA 1- RBC. Polypeptide bands were not detected on immunoblots using a monoclonal anti-DEA 1 antibody, therefore the anti-DEA 1 antibody may be specific for conformational epitopes lost during processing.<br />Conclusions: The autosomal dominant inheritance of DEA 1 with ≥ 4 alleles indicates a complex blood group system; the antigenicity of each DEA 1+ type will need to be determined. The biochemical nature of the DEA 1 antigen(s) appears different from human blood group systems tested.<br /> (© 2015 American Society for Veterinary Clinical Pathology.)
Details
- Language :
- English
- ISSN :
- 1939-165X
- Volume :
- 44
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Veterinary clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 26291052
- Full Text :
- https://doi.org/10.1111/vcp.12284