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Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats.

Authors :
Lee KH
Wang T
Jin YC
Lee SB
Oh JJ
Hwang JH
Lim JN
Lee JS
Lee HG
Source :
Journal of animal science and technology [J Anim Sci Technol] 2014 Jun 05; Vol. 56, pp. 6. Date of Electronic Publication: 2014 Jun 05 (Print Publication: 2014).
Publication Year :
2014

Abstract

The aims of study were to investigate the effects of intraperitoneal (i.p.) infusion of ghrelin on pancreatic α-amylase outputs and the responses of pancreatic proteins to ghrelin that may relate to the pancreatic exocrine. Six male Sprague-Dawley rats (300 g) were randomly divided into two groups, a control group (C, n = 3) and a treatment group (T, 10.0μg/kg BW, n = 3). Blood samples were collected from rat caudal vein once time after one hour injection. The concentrations of plasma ghrelin, cholecystokinin (CCK) and alfa-amylase activity were evaluated by enzyme immunoassay (EIA) kit. Two-dimensional gel electrophoresis (2-DE) analysis was conducted to separate the proteins in pancreas tissue. Results showed that the i.p. infusion of ghrelin at doses of 10.0 μg/kg body weight (BW) increased the plasma ghrelin concentrations (p = 0.07) and elevated the plasma CCK level significantly (p < 0.05). Although there was no statistically significant, the α-amylase activity tended to increase. The proteomics analysis indicated that some pancreatic proteins with various functions were up- or down- regulated compared with control group. In conclusion, ghrelin may have role in the pancreatic exocrine, but the signaling pathway was still not clear. Therefore, much more functional studies focus on these found proteins are needed in the near future.

Details

Language :
English
ISSN :
2055-0391
Volume :
56
Database :
MEDLINE
Journal :
Journal of animal science and technology
Publication Type :
Academic Journal
Accession number :
26290695
Full Text :
https://doi.org/10.1186/2055-0391-56-6