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Female spontaneously diabetic Torii fatty rats develop nonalcoholic steatohepatitis-like hepatic lesions.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2015 Aug 14; Vol. 21 (30), pp. 9067-78. - Publication Year :
- 2015
-
Abstract
- Aim: To investigate the histological features of the liver in spontaneously diabetic Torii (SDT) fatty rats compared with age-matched Sprague-Dawley (SD) rats.<br />Methods: Female SDT Lepr(fa) (SDT fatty) rats and age-matched SD rats were fed ad libitum. Body weight and biochemical parameters, such as serum glucose, triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels as well as fatty acid and TG accumulation in the liver were evaluated at 8 wk of age in the non-fasting state and at 8-wk intervals from 8 to 40 wk of age. Histopathological examinations of the liver were performed using hematoxylin and eosin and Sirius Red staining as well as double staining for ED-1 and toluidine blue. The expression of genes involved in TG synthesis, inflammation, and fibrosis was examined in the liver.<br />Results: SDT fatty rats showed significantly increased body weight compared with SD rats. Serum glucose, TG, and TC levels were significantly higher in SDT fatty rats compared with SD rats. The serum AST and ALT levels in SDT fatty rats were significantly elevated at 8 wk of age compared with the levels in SD rats. Hepatic TG content was marked in SDT fatty rats from 8 to 32 wk of age. Histopathologically, severe hepatosteatosis accompanied by inflammation was observed at 8 wk of age, and fibrosis started to occur at 32 wk of age. Furthermore, Sirius Red and ED-1 staining were increased in the liver at 32 wk of age. Hepatic gene expression related to TG synthesis, inflammation and fibrosis tended to increase in SDT fatty rats compared with SD rats, and the gene expression related to TG secretion was decreased in SDT fatty rats compared with SD rats.<br />Conclusion: Female SDT fatty rats have the potential to become an important animal model of nonalcoholic steatohepatitis with type 2 diabetes and obesity.
- Subjects :
- Age Factors
Alanine Transaminase blood
Animals
Aspartate Aminotransferases blood
Biomarkers blood
Blood Glucose metabolism
Diabetes Mellitus, Type 2 blood
Diabetes Mellitus, Type 2 genetics
Disease Models, Animal
Female
Gene Expression Regulation
Lipids blood
Liver enzymology
Liver Cirrhosis, Experimental pathology
Non-alcoholic Fatty Liver Disease blood
Non-alcoholic Fatty Liver Disease genetics
Obesity blood
Obesity genetics
RNA, Messenger metabolism
Rats, Inbred Strains
Rats, Sprague-Dawley
Severity of Illness Index
Sex Factors
Species Specificity
Weight Gain
Diabetes Mellitus, Type 2 complications
Liver pathology
Non-alcoholic Fatty Liver Disease etiology
Non-alcoholic Fatty Liver Disease pathology
Obesity complications
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 21
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 26290633
- Full Text :
- https://doi.org/10.3748/wjg.v21.i30.9067