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Hydrophobically Modified Keratin Vesicles for GSH-Responsive Intracellular Drug Release.
- Source :
-
Bioconjugate chemistry [Bioconjug Chem] 2015 Sep 16; Vol. 26 (9), pp. 1900-7. Date of Electronic Publication: 2015 Aug 26. - Publication Year :
- 2015
-
Abstract
- Redox-responsive polymersomes were prepared by self-assembly of a hydrophobically modified keratin and employing a water addition/solvent evaporation method. Polyethylene glycol-40 stearate (PEG40ST) was chosen as hydrophobic block to be coupled to keratin via radical grafting. The amphiphilic polymer exhibited low critical aggregation concentration (CAC; 10 μg/mL), indicating a good thermodynamic stability. The polymeric vesicles loaded both hydrophilic methotrexate and hydrophobic curcumin with high entrapment efficiencies, and showed a GSH-dependent drug release rate. Confocal studies on HeLa cells revealed that the obtained polymersomes were efficiently internalized. Biocompatibility properties of the proposed delivery vehicle were assessed in HET-CAM test and Balb-3T3 mouse fibroblasts. Polymersomes loaded with either methotrexate or curcumin inhibited HeLa and CHO-K1 cancer cells proliferation. Overall, the proposed keratin polymersomes could be efficient nanocarriers for chemotherapeutic agents.
- Subjects :
- 3T3 Cells
Animals
Biological Transport
CHO Cells
Cell Survival drug effects
Cricetinae
Cricetulus
Drug Carriers metabolism
Drug Carriers toxicity
Drug Stability
Glutathione metabolism
Glutathione toxicity
HeLa Cells
Humans
Mice
Thermodynamics
Drug Carriers chemistry
Drug Liberation
Glutathione chemistry
Hydrophobic and Hydrophilic Interactions
Intracellular Space metabolism
Keratins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-4812
- Volume :
- 26
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Bioconjugate chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26287808
- Full Text :
- https://doi.org/10.1021/acs.bioconjchem.5b00289