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MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium.
- Source :
-
Infection and immunity [Infect Immun] 2015 Nov; Vol. 83 (11), pp. 4266-76. Date of Electronic Publication: 2015 Aug 17. - Publication Year :
- 2015
-
Abstract
- MdsABC is a Salmonella-specific tripartite efflux pump that has been implicated in the virulence of Salmonella enterica serovar Typhimurium; however, little is known about the virulence factors associated with this pump. We observed MdsABC expression-dependent alterations in the degree of resistance to extracellular oxidative stress and macrophage-mediated killing. Thin-layer chromatography and tandem mass spectrometry analyses revealed that overexpression of MdsABC led to increased secretion of 1-palmitoyl-2-stearoyl-phosphatidylserine (PSPS), affecting the ability of the bacteria to invade and survive in host cells. Overexpression of MdsABC and external addition of PSPS similarly rendered the mdsABC deletion strain resistant to diamide. Diagonal gel analysis showed that PSPS treatment reduced the diamide-mediated formation of disulfide bonds, particularly in the membrane fraction of the bacteria. Salmonella infection of macrophages induced the upregulation of MdsABC expression and led to an increase of intracellular bacterial number and host cell death, similar to the effects of MdsABC overexpression and PSPS pretreatment on the mdsABC deletion strain. Our study shows that MdsABC mediates a previously uncharacterized pathway that involves PSPS as a key factor for the survival and virulence of S. Typhimurium in phagocytic cells.<br /> (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Bacterial Proteins genetics
Gene Expression Regulation, Bacterial
Humans
Macrophages metabolism
Macrophages microbiology
Membrane Transport Proteins genetics
Salmonella typhimurium genetics
Virulence
Bacterial Proteins metabolism
Membrane Transport Proteins metabolism
Salmonella Infections microbiology
Salmonella typhimurium metabolism
Salmonella typhimurium pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 83
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 26283336
- Full Text :
- https://doi.org/10.1128/IAI.00653-15