Back to Search
Start Over
Introduction to the diagnosis and classification of monocytic-lineage leukemias by flow cytometry.
- Source :
-
Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2017 May; Vol. 92 (3), pp. 218-227. Date of Electronic Publication: 2015 Aug 18. - Publication Year :
- 2017
-
Abstract
- Despite diagnostic criteria are currently available for the distinct subtypes of monocytic-lineage neoplasias, a number of partially overlapping features still remain evident, which may hamper their differential diagnosis. An accurate identification and characterization of monocytic cells is of major relevance for the diagnosis and classification of these neoplasias. In this regard, as compared to other conventional techniques, flow cytometry has shown the highest sensitivity for detection of early monocytic commitment of (normal and neoplastic) bone marrow CD34 <superscript>+</superscript> hematopoietic precursors as well as of monocytic aberrations and maturation blockades, which are frequently associated with clonal myeloid disorders. In the present paper we provide basic principles and criteria for multiparameter flow cytometry identification and characterization of bone marrow monocytic cells that contribute to an improved diagnostic and classification of monocytic lineage-associated acute leukemias in clinical settings, particularly when using the EuroFlow antibody panels. © 2015 International Clinical Cytometry Society.<br /> (© 2015 International Clinical Cytometry Society.)
- Subjects :
- Bone Marrow Cells pathology
Cell Differentiation genetics
Cell Lineage
Diagnosis, Differential
Hematopoietic Stem Cells
Humans
Leukemia, Monocytic, Acute blood
Leukemia, Monocytic, Acute pathology
Neoplasms blood
Neoplasms pathology
Flow Cytometry methods
Leukemia, Monocytic, Acute diagnosis
Monocytes pathology
Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1552-4957
- Volume :
- 92
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cytometry. Part B, Clinical cytometry
- Publication Type :
- Academic Journal
- Accession number :
- 26282340
- Full Text :
- https://doi.org/10.1002/cyto.b.21219