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The occurrence of Anaplasma phagocyto- philum in wild bison from the Bialowieza Primeval Forest in Eastern Poland.
- Source :
-
Berliner und Munchener tierarztliche Wochenschrift [Berl Munch Tierarztl Wochenschr] 2015 Jul-Aug; Vol. 128 (7-8), pp. 310-4. - Publication Year :
- 2015
-
Abstract
- The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 183908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum.
Details
- Language :
- English
- ISSN :
- 0005-9366
- Volume :
- 128
- Issue :
- 7-8
- Database :
- MEDLINE
- Journal :
- Berliner und Munchener tierarztliche Wochenschrift
- Publication Type :
- Academic Journal
- Accession number :
- 26281444