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Identification and complete genome analysis of a novel bovine picornavirus in Japan.
- Source :
-
Virus research [Virus Res] 2015 Dec 02; Vol. 210, pp. 205-12. Date of Electronic Publication: 2015 Aug 07. - Publication Year :
- 2015
-
Abstract
- We identified novel viruses in feces from cattle with diarrhea collected in 2009 in Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the (near) complete sequences of the virus. Sequence analyses revealed that they had a standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1 (VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group 1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9% (P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses and detailed genome characterization showed that they, together with the unclassified Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding regions. These viruses had conserved features (e.g. predicted protein cleavage sites, presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they have a common ancestor. Reverse-transcription-PCR assays, using specific primers designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal samples from cattle with diarrhea were positive, indicating the prevalence of these picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further studies are needed to investigate the pathogenic potential and etiological role of these viruses in cattle.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cattle
Cattle Diseases epidemiology
Cluster Analysis
Diarrhea epidemiology
Diarrhea veterinary
Diarrhea virology
Feces virology
Gene Order
Genes, Viral
Japan epidemiology
Molecular Sequence Data
Phylogeny
Picornaviridae genetics
Picornaviridae Infections virology
Prevalence
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology
Cattle Diseases virology
Genome, Viral
Picornaviridae classification
Picornaviridae isolation & purification
Picornaviridae Infections veterinary
RNA, Viral genetics
Sequence Analysis, DNA
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7492
- Volume :
- 210
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 26260333
- Full Text :
- https://doi.org/10.1016/j.virusres.2015.08.001