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Ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppressing nicotinic acetylcholine receptor-ion channels in cultured bovine adrenal medullary cells.
- Source :
-
Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2015 Dec; Vol. 388 (12), pp. 1259-69. Date of Electronic Publication: 2015 Aug 11. - Publication Year :
- 2015
-
Abstract
- Ikarisoside A is a natural flavonol glycoside derived from plants of the genus Epimedium, which have been used in Traditional Chinese Medicine as tonics, antirheumatics, and aphrodisiacs. Here, we report the effects of ikarisoside A and three other flavonol glycosides on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. We found that ikarisoside A (1-100 μM), but not icariin, epimedin C, or epimedoside A, concentration-dependently inhibited the secretion of catecholamines induced by acetylcholine, a physiological secretagogue and agonist of nicotinic acetylcholine receptors. Ikarisoside A had little effect on catecholamine secretion induced by veratridine and 56 mM K(+). Ikarisoside A (1-100 μM) also inhibited (22)Na(+) influx and (45)Ca(2+) influx induced by acetylcholine in a concentration-dependent manner similar to that of catecholamine secretion. In Xenopus oocytes expressing α3β4 nicotinic acetylcholine receptors, ikarisoside A (0.1-100 μM) directly inhibited the current evoked by acetylcholine. It also suppressed (14)C-catecholamine synthesis and tyrosine hydroxylase activity induced by acetylcholine at 1-100 μM and 10-100 μM, respectively. The present findings suggest that ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppression of nicotinic acetylcholine receptor-ion channels in bovine adrenal medullary cells.
- Subjects :
- Acetylcholine toxicity
Adrenal Medulla metabolism
Animals
Calcium Channels metabolism
Catecholamines biosynthesis
Catecholamines metabolism
Cattle
Cells, Cultured
Dose-Response Relationship, Drug
Female
Flavonoids isolation & purification
Glycosides isolation & purification
Ion Channel Gating physiology
Nicotinic Antagonists isolation & purification
Nicotinic Antagonists pharmacology
Plant Extracts isolation & purification
Plant Extracts pharmacology
Plant Leaves
Sodium Channels metabolism
Xenopus laevis
Acetylcholine antagonists & inhibitors
Adrenal Medulla drug effects
Catecholamines antagonists & inhibitors
Flavonoids pharmacology
Glycosides pharmacology
Ion Channel Gating drug effects
Receptors, Nicotinic metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1912
- Volume :
- 388
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Naunyn-Schmiedeberg's archives of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26257152
- Full Text :
- https://doi.org/10.1007/s00210-015-1161-y