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Distinct Regulation of Transmitter Release at the Drosophila NMJ by Different Isoforms of nemy.
- Source :
-
PloS one [PLoS One] 2015 Aug 03; Vol. 10 (8), pp. e0132548. Date of Electronic Publication: 2015 Aug 03 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Synaptic transmission is highly plastic and subject to regulation by a wide variety of neuromodulators and neuropeptides. In the present study, we have examined the role of isoforms of the cytochrome b561 homologue called no extended memory (nemy) in regulation of synaptic strength and plasticity at the neuromuscular junction (NMJ) of third instar larvae in Drosophila. Specifically, we generated two independent excisions of nemy that differentially affect the expression of nemy isoforms. We show that the nemy45 excision, which specifically reduces the expression of the longest splice form of nemy, leads to an increase in stimulus evoked transmitter release and altered synaptic plasticity at the NMJ. Conversely, the nemy26.2 excision, which appears to reduce the expression of all splice forms except the longest splice isoform, shows a reduction in stimulus evoked transmitter release, and enhanced synaptic plasticity. We further show that nemy45 mutants have reduced levels of amidated peptides similar to that observed in peptidyl-glycine hydryoxylating mono-oxygenase (PHM) mutants. In contrast, nemy26.2 mutants show no defects in peptide amidation but rather display a decrease in Tyramine β hydroxylase activity (TβH). Taken together, these results show non-redundant roles for the different nemy isoforms and shed light on the complex regulation of neuromodulators.
- Subjects :
- Animals
Animals, Genetically Modified
Cytochrome b Group genetics
Drosophila Proteins genetics
Drosophila melanogaster
Neuronal Plasticity physiology
Protein Isoforms genetics
Cytochrome b Group metabolism
Drosophila Proteins metabolism
Neuromuscular Junction metabolism
Presynaptic Terminals metabolism
Protein Isoforms metabolism
Synaptic Transmission physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26237434
- Full Text :
- https://doi.org/10.1371/journal.pone.0132548