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The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres.
- Source :
-
PLoS genetics [PLoS Genet] 2015 Jul 31; Vol. 11 (7), pp. e1005410. Date of Electronic Publication: 2015 Jul 31 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Dyskeratosis Congenita (DC) is a heritable multi-system disorder caused by abnormally short telomeres. Clinically diagnosed by the mucocutaneous symptoms, DC patients are at high risk for bone marrow failure, pulmonary fibrosis, and multiple types of cancers. We have recapitulated the most common DC-causing mutation in the shelterin component TIN2 by introducing a TIN2-R282H mutation into cultured telomerase-positive human cells via a knock-in approach. The resulting heterozygous TIN2-R282H mutation does not perturb occupancy of other shelterin components on telomeres, result in activation of telomeric DNA damage signaling or exhibit other characteristics indicative of a telomere deprotection defect. Using a novel assay that monitors the frequency and extension rate of telomerase activity at individual telomeres, we show instead that telomerase elongates telomeres at a reduced frequency in TIN2-R282H heterozygous cells; this recruitment defect is further corroborated by examining the effect of this mutation on telomerase-telomere co-localization. These observations suggest a direct role for TIN2 in mediating telomere length through telomerase, separable from its role in telomere protection.
- Subjects :
- Aminopeptidases metabolism
Cell Line, Tumor
DNA Repair genetics
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases metabolism
Dyskeratosis Congenita genetics
Gene Knock-In Techniques
HCT116 Cells
Humans
Mutation genetics
Serine Proteases metabolism
Shelterin Complex
Telomere Homeostasis genetics
Telomeric Repeat Binding Protein 1 genetics
Tripeptidyl-Peptidase 1
Telomerase metabolism
Telomere metabolism
Telomere Shortening genetics
Telomere-Binding Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 11
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 26230315
- Full Text :
- https://doi.org/10.1371/journal.pgen.1005410