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Sphingopyxis flava sp. nov., isolated from a hexachlorocyclohexane (HCH)-contaminated soil.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2015 Oct; Vol. 65 (10), pp. 3720-3726. Date of Electronic Publication: 2015 Jul 26. - Publication Year :
- 2015
-
Abstract
- A Gram-negative-staining, aerobic, non-motile, non-spore-forming, rod-shaped and yellow-pigmented bacterium, designated R11HT, was isolated from a soil sample collected from a hexachlorocyclohexane dumpsite located at Ummari village, Lucknow, Uttar Pradesh, India. The 16S rRNA gene sequence similarity between strain R11HT and the type strains of species of genus Sphingopyxis with validly published names ranged from 93.75 to 97.85 %. Strain R11HT showed the highest 16S rRNA gene sequence similarity to Sphingopyxis indica DS15T (97.85 %), followed by Sphingopyxis soli JCM15910T (97.79 %), Sphingopyxis ginsengisoli KCTC 12582T (97.77 %) and Sphingopyxis panaciterrulae KCTC 22112T (97.34 %). The DNA G+C content of strain R11HT was 63.5 mol%. DNA-DNA relatedness between strain R11HT and its closest phylogenetic neighbours was well below the threshold value of 70 %, which suggested that strain R11HT represents a novel species of the genus Sphingopyxis. The major polar lipids of strain R11HT were sphingoglycolipid and other lipids commonly reported in this genus, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. Spermidine was detected as the major polyamine. The chemotaxonomic markers in strain R11HT confirmed its classification in the genus Sphingopyxis, i.e. Q-10 as the major ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C14 : 0 2-OH as the predominant fatty acids. Results obtained from DNA-DNA hybridization and chemotaxonomic and phenotypic analyses clearly distinguished strain R11HT from its closest phylogenetic neighbours. Thus, strain R11HT represents a novel species of the genus Sphingopyxis, for which the name Sphingopyxis flava sp. nov. is proposed. The type strain is R11HT ( = DSM 28472T = MCC 2778T).
- Subjects :
- Bacterial Typing Techniques
Base Composition
DNA, Bacterial genetics
Fatty Acids chemistry
India
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids chemistry
Pigmentation
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Soil Pollutants analysis
Spermidine chemistry
Sphingomonadaceae genetics
Sphingomonadaceae isolation & purification
Ubiquinone analogs & derivatives
Ubiquinone chemistry
Hexachlorocyclohexane analysis
Phylogeny
Soil Microbiology
Sphingomonadaceae classification
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 65
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26219279
- Full Text :
- https://doi.org/10.1099/ijsem.0.000482