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Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen.
- Source :
-
Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2015 Sep; Vol. 6 (6), pp. 820-8. Date of Electronic Publication: 2015 Jul 20. - Publication Year :
- 2015
-
Abstract
- Ticks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.<br /> (Copyright © 2015 Elsevier GmbH. All rights reserved.)
- Subjects :
- Adjuvants, Immunologic
Animals
Antibodies blood
Cell Membrane chemistry
Escherichia coli
Female
Mice
Mice, Inbred BALB C
Rabbits
Swine
Anaplasma marginale immunology
Antigens immunology
Arthropod Proteins immunology
Bacterial Outer Membrane Proteins immunology
Rhipicephalus immunology
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1877-9603
- Volume :
- 6
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Ticks and tick-borne diseases
- Publication Type :
- Academic Journal
- Accession number :
- 26219233
- Full Text :
- https://doi.org/10.1016/j.ttbdis.2015.07.010