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Association between hepatitis B virus and MHC class I polypeptide-related chain A in human hepatocytes derived from human-mouse chimeric mouse liver.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 Sep 04; Vol. 464 (4), pp. 1192-1195. Date of Electronic Publication: 2015 Jul 23. - Publication Year :
- 2015
-
Abstract
- Due to the lack of efficient hepatitis B virus (HBV) infection systems, progress in understanding the role of innate immunity in HBV infection has remained challenging. Here we used human hepatocytes from a humanized severe combined immunodeficiency albumin promoter/enhancer driven-urokinase-type plasminogen activator mouse model for HBV infection. HBV DNA levels in culture medium from these human hepatocytes were 4.8-5.7 log IU/mL between day 16 and day 66 post-infection by HBV genotype C inoculum. HBV surface antigen (HBsAg) was also detected by chemiluminescent immunoassay from day 7 to day 66 post-infection. Western blot analysis revealed that major histocompatibility complex class I-related chain A (MICA), which plays a role in the innate immune system, was induced in HBV-infected human hepatocytes 27 days after infection compared with the uninfected control. MICA was reduced at day 62 and undetectable at day 90. Of interest, MICA expression by human hepatocytes increased after HBV infection and decreased before HBsAg loss. Human hepatocytes derived from chimeric mice with hepatocyte-humanized liver could support HBV genome replication. Further studies of the association between HBV replication and MICA induction should be conducted.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cells, Cultured
Chimera immunology
Chimera virology
Hepatitis B virus isolation & purification
Humans
Liver cytology
Liver virology
Mice
Mice, SCID
Hepatitis B immunology
Hepatitis B virology
Hepatitis B virus physiology
Hepatocytes immunology
Hepatocytes virology
Histocompatibility Antigens Class I immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 464
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 26212443
- Full Text :
- https://doi.org/10.1016/j.bbrc.2015.07.102