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Intravenous Preload of Mesenchymal Stem Cells Rescues Erectile Function in a Rat Model of Cavernous Nerve Injury.
- Source :
-
The journal of sexual medicine [J Sex Med] 2015 Aug; Vol. 12 (8), pp. 1713-21. Date of Electronic Publication: 2015 Jul 24. - Publication Year :
- 2015
-
Abstract
- Introduction: We evaluated the potential preventive effects and mechanisms of intravenously preloaded mesenchymal stem cells (MSCs) for erectile dysfunction (ED) in a cavernous nerve (CN) injury model.<br />Methods: Male Sprague-Dawley (SD) rats were used for this study. Rats were randomized into two groups. One group was intravenously preloaded with MSCs (1.0 × 10(6) cells in 1 mL total fluid volume) and the other was infused with medium alone (1 mL Dulbecco's modified Eagle's medium [DMEM]) for sham control, respectively. Crushed CN injury was induced immediately after infusion. The surgeon was blind to the experimental conditions (MSC or medium).<br />Main Outcome Measures: To assess erectile function, we measured the intracavernous pressure (ICP) and arterial pressure (AP) at 1 hour and 2 weeks after CN injury. After measuring the initial ICP/AP of pre-injury (normal) male SD rats, they were randomized into the two groups and infused with MSCs or medium. PKH26-labelled MSCs were used for tracking. To investigate the mRNA expression levels of neurotrophins in the major pelvic ganglia (MPG), we performed real-time quantitative real-time polymerase chain reaction.<br />Results: The reduction of ICP/AP and area under the curve of ICP (ICP-AUC) in the MSC group was significantly lower than in the DMEM group (P < 0.05; P < 0.05) at 1 hour. The ICP/AP and ICP-AUC at 2 weeks post-injury in the MSC group was significantly higher than in the DMEM group (P < 0.01; P < 0.05). The preloaded PKH26-labelled MSCs were detected in the MPG and CN using confocal microscopy indicating homing of the cells to the injured nerve and ganglia. Glia cell-derived neurotrophic factor (GDNF) and neurturin, which are important neurotrophic factors for erection, had expression levels in MPG significantly higher in the MSC group than in the DMEM group (P < 0.01, 0.05).<br />Conclusion: Intravenous preload of MSCs before a CN injury may prevent or reduce experimental ED.<br /> (© 2015 International Society for Sexual Medicine.)
- Subjects :
- Animals
Disease Models, Animal
Erectile Dysfunction therapy
Glial Cell Line-Derived Neurotrophic Factor
Hypogastric Plexus metabolism
Male
Nerve Crush
Nerve Regeneration
Neurturin
Penile Erection physiology
Penis innervation
Rats
Rats, Sprague-Dawley
Erectile Dysfunction pathology
Ganglia pathology
Penile Erection drug effects
Penis pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1743-6109
- Volume :
- 12
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The journal of sexual medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26211660
- Full Text :
- https://doi.org/10.1111/jsm.12957